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Hi Miguel/Schores, I have the same issue with artefacts apparent in unfiltered half maps in relion, this time with a helix (see attached). I've tried changing the references, and the .star file, and a number of other things including rextracting the particles. I using relion v2.1.0. Have we identified any way around this? Cheers, Joe ######################################################################## To unsubscribe from the CCPEM list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCPEM&A=1