 |
 |
Dear all, Thanks a lot. Apparently, for whatever reason, each image has slightly different number of pixels (varing from 4089 to 4096). Is there a clipping or cropping function in Relion to make all images in the same number of pixels? Best wishes, Jeongyoon > On 18 Jul 2018, at 07:57, Sjors Scheres <[log in to unmask]> wrote: > > Hi Jeongyoon, > Perhaps you could try to run relion_image_handler --stats on the > micrographs.star file (you may need to change the rlnMicrographName label > into rlnImageName for this). Just to make sure there is nothing unexpected > there.. > HTH, > Sjors > >> Dear all, >> >> I’m trying to perform auto-picking of 100 micrographs containing 4096 >> pixels on each side and all are in the same magnification. >> I’m sure the images are in the same size, but when I ran the >> auto-picking I got these errors as attached here. >> Does anyone get the same problem as me? Or anyone could help me in this >> please? >> It seems the program tries to change the pixel size for whatever reason?? >> >> Thanks a lot, >> Jeongyoon >> >> [cid:7A1B2F1B-D0D4-4F94-BE39-E11A885E5595@bioch.ox.ac.uk] >> >> ######################################################################## >> >> To unsubscribe from the CCPEM list, click the following link: >> https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCPEM&A=1 >> > > > -- > Sjors Scheres > MRC Laboratory of Molecular Biology > Francis Crick Avenue, Cambridge Biomedical Campus > Cambridge CB2 0QH, U.K. > tel: +44 (0)1223 267061 > http://www2.mrc-lmb.cam.ac.uk/groups/scheres > ######################################################################## To unsubscribe from the CCPEM list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCPEM&A=1