Look at the scl_slope and scl_intercept in the NIfTI headers, which should (for these data) have values of 1 and 0. The mnc2nii software used to convert these images seems to have put random tiny values in them. . The header contents are below: sizeof_hdr: 348 data_type: ' ' db_name: ' ' extents: 0 session_error: 0 regular: 'r' dim_info: 0 dim: [3 197 233 189 0 0 0 0] intent_p1: 0 intent_p2: 0 intent_p3: 0 intent_code: 0 datatype: 16 bitpix: 32 slice_start: 0 pixdim: [0 1 1 1 0 1 0 0] vox_offset: 348 scl_slope: 1.5259e-05 scl_inter: -2.7413e-18 slice_end: 0 slice_code: 0 xyzt_units: 10 cal_max: 0 cal_min: 0 slice_duration: 0 toffset: 0 glmax: 0 glmin: 0 descrip: 'mnc2nii nihpd_sym_04.5-08.5_gm.mnc nihpd_sym_04.5-08.5_gm.nii ' aux_file: ' ' qform_code: 0 sform_code: 1 quatern_b: 0 quatern_c: 0 quatern_d: 0 qoffset_x: 0 qoffset_y: 0 qoffset_z: 0 srow_x: [1 0 0 -98] srow_y: [0 1 0 -134] srow_z: [0 0 1 -72] intent_name: ' ' magic: 'n+1 ' Best regards, -John On Wed, 20 Jun 2018 at 23:05, avantika mathur <[log in to unmask]> wrote: > Dear SPM users, > > I am working on fMRI data from 5-6 year old children. I wanted to use a > pediatric template for normalization. I downloaded the *4.5–8.5 y.o* > (prepuberty) NIHPD template from (http://nist.mni.mcgill.ca/?p=974). > Thereafter, I converted the files to .nii format. Visualization of nii > files reveal perfectly ffine images (Attached is the template). > nihpd_sym_04.5-08.5_csf.nii > <https://drive.google.com/file/d/1VImQKu0EFWMwwyTzAYA3FHULUdFHEV5J/view?usp=drive_web> > > nihpd_sym_04.5-08.5_gm.nii > <https://drive.google.com/file/d/1EcRkVuDAa05yUjpjNnuBvUxfzkcG8EZA/view?usp=drive_web> > > nihpd_sym_04.5-08.5_mask.nii > <https://drive.google.com/file/d/1UUmRnL6A_tk2hu6mj-CHoA97E0m4H_ba/view?usp=drive_web> > > nihpd_sym_04.5-08.5_pdw.nii > <https://drive.google.com/file/d/1D7HGV6MdJ-p_eM2R77aGAw6cS_F61Dh2/view?usp=drive_web> > > nihpd_sym_04.5-08.5_t1w.nii > <https://drive.google.com/file/d/1FgB6YWYdKGBJwYaEvOQXI7xjVr1X2vWo/view?usp=drive_web> > > nihpd_sym_04.5-08.5_t2w.nii > <https://drive.google.com/file/d/1jnBevSS_FTn1eMkPigYgqKppDfspVQfn/view?usp=drive_web> > > nihpd_sym_04.5-08.5_wm.nii > <https://drive.google.com/file/d/1Yjkshq9wnWTGMMcLkLe0I1i0a3ttrCke/view?usp=drive_web> > > > I am using old segment in SPM12. I enter the subject data T1 image (Data) > and tissue probability maps from the template (grey matter, white matter > and csf ). Once I run segment the output files c1 (grey matter), c2 (white > matter) and c3 (csf) are empty. The segmentation fails giving me null > images. > > Does anyone know why this happens? > > What tissue probability maps should I use for segmentation (old segment) > in pediatric population? Then how do I use the output in normalization? > > Please let me know if anyone has faced the same issue before. > > Thanks > > -- > Avantika Mathur > -- Prof John Ashburner Professor of Imaging Science UCL Institute of Neurology Queen Square Wellcome Trust Centre for Neuroimaging University College London 12 Queen Square, London, WC1N 3BG E: [log in to unmask] T: +44 (0)20 3448 4365 http://www.fil.ion.ucl.ac.uk/