Hi Amy,
interesting you should mention this: We've recently started seeing more discrepancies between our A.fumigatus precipitating antibody assay (counter-current immunoelectrophoresis method) and the A.fumigatus IgG FEIA on the ImmunoCAP. I'll investigate whether it coincides with these lot numbers and let you know.
Basically we're seeing mild/moderately elevated A.fumigatus specific IgG results on the ImmunoCAP (values typically 50-100) with completely negative CIE results (tested for precipitins to both A.fumigatus filtrate and somatic antigens). We would expect negative CIE results to generate a corresponding A.fumigatus IgG result <40.
thanks,
Adrian
Dr Adrian Heaps BSc MSc PhD DIC FRCPath
Consultant Clinical Scientist & Clinical Lead for Immunology
Department of Virology & Immunology
Cumberland Infirmary
Newtown Road
Carlisle
Cumbria
CA2 7HY
Cumberland Infirmary contact:
Tel: 01228 814367 / 814645
Fax: 01228 814864
CIC bleep 704
The NCUHT Pathology Handbook can be found here
James Cook University Hospital contact:
Tel: 01642 854129 / 835642
** Please note I work at the RVI Newcastle on Mondays **
Royal Victoria Infirmary, Newcastle contact:
Hi all,
We have switched to a new lot of Immunocap specific IgA/IgG sample diluent (box lot no. L06U, bottle lot no. AJLCH), and noticed an approximate 20% increase in our IgG Aspergillus precipitins in-house pooled patient QC as well as patient/EQA results. There has not been a shift in the kit QCs.
Has anyone else moved to this new lot and seen a similar shift?
Thanks in advance,
Amy
Amy Kiley
Principal Clinical Biochemist
Clinical Laboratory Sciences
Doncaster and Bassetlaw Teaching Hospitals NHS Foundation Trust
Doncaster Royal Infirmary, Armthorpe Road, Doncaster DN2 5LT
|
01302 642841 |
This email is confidential, private and privileged. If you are not the intended recipient please accept our apologies. Do not disclose copy or distribute information in this email or take any action in reliance on its contents; to do so is strictly prohibited and may be unlawful. Please inform us that this email has gone astray before deleting it. Thank you for your cooperation.