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Dear Sutirtha, this is a great question. In fact, as far as I know, blood gas analysis is greatly affected in the case that a tight airseal of the sample cannot be ensured. PCO2 diffuses out of the sample into the atmosphere, leading to increases in pH. The bicarb in the sample is anyway calculated through pH and pCO2 und would therefore also be affected. I hope this answers you question somewhat? Best regards, PD Dr. med. Orfeas Liangos Senior Physician, Division of Nephrology Klinikum Coburg, Germany Dr. Orfeas Liangos Nephrologie Oberarzt ------------------------------------------------------------------------------------------- REGIOMED-KLINIKEN GmbH Klinikum Coburg GmbH Ketschendorfer Str. 33 96450 Coburg E-Mail: [log in to unmask]<mailto:[log in to unmask]> Telefon: +49 9561 22 - 33304 Telefax: +49 9561 249611 Internet: www.regiomed-kliniken.de Folgen Sie uns auf: Facebook<https://www.facebook.com/regiomed> | Instagram<https://www.instagram.com/regiomed.kliniken> | YouTube<https://www.youtube.com/channel/UCOQLqoJEch1YlzF6rYYvhiQ> | Xing<https://www.xing.com/companies/regiomed-klinikengmbh> [cid:imagefce5ea.JPG@2b43ad38.42b3ba1c] *********************************************************************************************************************************************** Klinikum Coburg GmbH, Ketschendorfer Str. 33, D - 96450 Coburg Sitz und Registergericht Coburg HRB 3040 Geschäftsführer: Joachim Bovelet (Vorsitzender), Prof. Dr. Johannes Brachmann, Thomas Hergt Ein Unternehmen der REGIOMED-KLINIKEN GmbH *********************************************************************************************************************************************** Diese E-Mail und angehängte Dateien sind vertraulich und ausschließlich für den oder die als Empfänger eingetragenen Personen bestimmt. Sollten Sie diese Nachricht aufgrund eines Fehlers oder Irrtums erhalten haben, informieren Sie bitte den Systemverwalter und/oder den Absender der Nachricht. Diese Fußnote bestätigt auch, dass diese E-Mail auf Viren überprüft wurde. Dies stellt jedoch keine Garantie auf völlige Virenfreiheit dar. *********************************************************************************************************************************************** ________________________________ Von: Clinical biochemistry discussion list [[log in to unmask]]" im Auftrag von "Dr. Sutirtha Chakraborty [[log in to unmask]] Gesendet: Donnerstag, 21. Juni 2018 11:37 An: [log in to unmask] Betreff: venous blood gas Hi all, We do a lot of venous blood gas testing in our hospitals which we collect in lithium heparin tubes. I was wondering if analytes like pH, pCO2, Hb, electrolytes and Glucose could be affected by underfilled tubes? Specially interested to know pH and PCO2 are influenced? Regards, Sutirtha -- Sutirtha Chakraborty, MBBS, MD Seksjonsoverlege Laboratorium for Medisinsk Biokjemi Helse Fonna HF Haugesund, Norway www.helse-fonna.no<http://www.helse-fonna.no/> ------ACB discussion List Information-------- This is an open discussion list for the academic and clinical community working in clinical biochemistry. Please note, archived messages are public and can be viewed via the internet. Views expressed are those of the individual and they are responsible for all message content. ACB Web Site http://www.acb.org.uk Green Laboratories Work http://www.laboratorymedicine.nhs.uk List Archives http://www.jiscmail.ac.uk/lists/ACB-CLIN-CHEM-GEN.html List Instructions (How to leave etc.) http://www.jiscmail.ac.uk/ ------ACB discussion List Information-------- This is an open discussion list for the academic and clinical community working in clinical biochemistry. Please note, archived messages are public and can be viewed via the internet. Views expressed are those of the individual and they are responsible for all message content. ACB Web Site http://www.acb.org.uk Green Laboratories Work http://www.laboratorymedicine.nhs.uk List Archives http://www.jiscmail.ac.uk/lists/ACB-CLIN-CHEM-GEN.html List Instructions (How to leave etc.) http://www.jiscmail.ac.uk/