----------------------------------------

Dr. Tharin Blumenschein, Lecturer

Director of Employability

School of Chemistry

University of East Anglia

Norwich - NR4 7TJ

United Kingdom

Phone: +44 (0)1603 59 2963

 

University of East Anglia

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From: "CcpNmr software mailing list <[log in to unmask]>" <[log in to unmask]> on behalf of Nina Christou <[log in to unmask]>
Reply-To: "CcpNmr software mailing list <[log in to unmask]>" <[log in to unmask]>
Date: Thursday, 31 May 2018 13:51
To: "CcpNmr software mailing list <[log in to unmask]>" <[log in to unmask]>
Subject: Pseudo3D spectrum import problem

 

Dear Sir/Madam,

My name is Nina Christou and I'm currently using CCPN Analysis 2.4.2 to 

analyse some Pseudo 3D data I collected of an Hydrogen-Deuterium 

exchange experiment I recorded. It seems that the data are partly ruined 

when I import them in Bruker format so, as a result, I cannot perform my 

analysis.

Namely, I have a series of HN spectra collected (457 to be exact) 

overtime. I import them directly from the acquired spectra from a Bruker 

spectrometer. I have a similar set of data which I imported last week 

and everything worked perfectly. However, this time, it seems like the 

spectra going from plane to plane are different. I am attaching some 

pictures which show a series of some planes, from the lowest to the 

highest, so you can have an idea of what I'm talking about. I have a 

reference spectrum (pink) which should overlap with the mauve spectra 

(pseudo-3D). Going from the lowest to the highest plane, the intensity 

of the peaks should decrease but not shift.

As a result of this weird read out of my data (spectra is shifting and 

some peaks are 'halved'), instead of having a normal exponential decay 

of the intensity of each peak overtime, I get a graph starting at noise 

level intensity, followed by some zeros and eventually an exponential 

decay. I have included a graph of such a peak to aid to your understanding.

Can you let me know how I could potentially fix that? I have run out of 

ideas myself.

Sincerely,

Nina