This sounds reasonable to try yes, as long the symmetry to be applied can be seen in the classification volume.

However if the classification volume is good enough that its symmetry group can be seen, then the classification has probably worked quite well. In this case why not take the particles and volume into a refinement, with and without the symmetry imposed as a sanity check.

Best,
Kyle

On 23 May 2018, at 10:09, Hamidreza Rahmani <[log in to unmask]> wrote:

Hi, 

How about doijg the classification in C1, then apply symmetry on your class averages, then use them as the reference on another C1 classification. 

Would this help at all?
Best,
Hamid



Sent via the Samsung Galaxy S8, an AT&T 4G LTE smartphone

-------- Original message --------
From: Kyle Morris <[log in to unmask]>
Date: 5/23/18 12:51 PM (GMT-05:00)
To: [log in to unmask]
Subject: Re: [ccpem] MIXSymmetry in 3Dclassification

Hi Melisa,

Sounds like an interesting problem!

Be very careful here, assuming any kind of symmetry during classification could lead to spurious classification results.

It would probably be best to stay in C1/asymmetric and you hope those particles with symmetry should still classify and separate from those without symmetry. Once you have the classes separated in C1 and can see the symmetry in those volumes, then you can safely apply symmetry in further processing.

Good luck!
Kyle

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Kyle L. Morris PhD
Postdoctoral Scholar
Hurley Lab
QB3 Stanley Hall 
UCB

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On 23 May 2018, at 03:42, Melisa Lázaro <[log in to unmask]> wrote:

Dear EM community,

I was wondering if there is any possibility to perform  3D classification to a set of particles and apply different symmetries for each class. In this way one could separate symmetric from asymmetric particles.
Thank you in advance.

 

 

Center of Excellence Severo Ochoa
Center of Excellence Severo Ochoa (2017-2021)
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