Hi,How about doijg the classification in C1, then apply symmetry on your class averages, then use them as the reference on another C1 classification.Would this help at all?Best,HamidSent via the Samsung Galaxy S8, an AT&T 4G LTE smartphoneHi Melisa,-------- Original message --------From: Kyle Morris <[log in to unmask]>Date: 5/23/18 12:51 PM (GMT-05:00)Subject: Re: [ccpem] MIXSymmetry in 3DclassificationSounds like an interesting problem!Be very careful here, assuming any kind of symmetry during classification could lead to spurious classification results.It would probably be best to stay in C1/asymmetric and you hope those particles with symmetry should still classify and separate from those without symmetry. Once you have the classes separated in C1 and can see the symmetry in those volumes, then you can safely apply symmetry in further processing.Good luck!Kyle,-,-,-,-,-,-,-,-,-,-,-,-,-,-,-,-,-,-,-,-,-,-,-,-,-,-,-,-,-,-,-,-,-‘-‘-‘-‘-‘-‘-‘-‘-‘-‘-‘-‘-‘-‘-‘-‘-‘-‘-‘-‘-‘-‘-‘-‘-‘-‘-‘-‘-‘-‘-‘-‘-‘-‘-‘-‘-,-,-,-,-,-,-,-,-,-,-,-,-,-,-,-,-,-,-,-,-,-,-,-,-,-,-,-,-,-,-,-,-,-‘-‘-‘-‘-‘-‘-‘-‘-‘-‘-‘-‘-‘-‘-‘-‘-‘-‘-‘-‘-‘-‘-‘-‘-‘-‘-‘-‘-‘-‘-‘-‘-‘-‘-‘-‘-On 23 May 2018, at 03:42, Melisa Lázaro <[log in to unmask]> wrote:Dear EM community,I was wondering if there is any possibility to perform 3D classification to a set of particles and apply different symmetries for each class. In this way one could separate symmetric from asymmetric particles.Thank you in advance.
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