Hi, first maps are produced by Refmac, not Coot, and second it shouldn't be called sigma because it's not an uncertainty, it's a root-mean-square deviation from the mean. The equation for the RMSD can be found in any basic text on statistics, e.g. just type 'RMSD' in Wikipedia. Cheers -- Ian On 19 April 2018 at 13:20, Mohamed Ibrahim <[log in to unmask]> wrote: > Dear COOT users, > > Do you know how to extract the equations that COOT uses for generating the > maps and calculating the sigma values? > > Best regards, > Mohamed > > -- > > -- > *----------------------------------* > *Mohamed Ibrahim * > > *Humboldt University * > *Berlin, Germany * > > *Tel: +49 30 209347931 * >