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Dear Hermann Could you please advise where you are based, and I can then get the appropriate Waters specialists to contact you to discuss the options. Best regards Jas Mahey TA Instruments - a Division of Waters Ltd. -----Original Message----- From: CCP4 bulletin board <[log in to unmask]> On Behalf Of Markus Heckmann Sent: 26 April 2018 17:04 To: [log in to unmask] Subject: [ccp4bb] size exclusion columns Dear all, We are looking for a size exclusion chromatography column (silica-based) for protein purification prior to a MALS-detector. We looked for (www.waters.com BEH-450), Sepax (Unix-C 300) and Phenomex (BioZen SEC-3). Any 'column' tips or recommendations when dealing with large proteins (MDa)? Many thanks Markus