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Dears, everybody knows that B-factors may change amongst different crystal structures and that they need to be standardized when different protein crystal structures are compared. If I am not wrong, I remember that someone proposed to standardize B-factors of protein atoms as “BS = B - Bave”, where Bave is the average B-factor of the protein. Such standardization is based on the hypothesis that independent sources of disorder add in determining the final B-factor. BS should represent atomic B-factors depurated by all factors different from atom oscillation, since Bave differences are neutralized. Does anyone can help me in finding a publication (80s or 90s) where these BS values are used? Thanks! Oliviero