Dear experts, I am analyzing two data sets that were collected using multi-band, and not sure how to enter the timing information during the slice timing and first-level estimation steps. In the first study, the TR is 500 ms, and I have got the acquisition times below: >> hdr1 = spm_dicom_headers(xDicomFile{1}); >> hdr1{1}.Private_0019_1029' ans = 0 180.0000 360.0000 60.0000 240.0000 420.0000 120.0000 300.0000 0 180.0000 360.0000 60.0000 240.0000 420.0000 120.0000 300.0000 0 180.0000 360.0000 60.0000 240.0000 420.0000 120.0000 300.0000 0 180.0000 360.0000 60.0000 240.0000 420.0000 120.0000 300.0000 0 180.0000 360.0000 60.0000 240.0000 420.0000 120.0000 300.0000 0 180.0000 360.0000 60.0000 240.0000 420.0000 120.0000 300.0000 I assume that these numbers should go to "Slice Timing - Slice order", and TR set to 0.5. My questions are: (1) Should "Slice Timing - TA" be *0.42*? (2) Can "Slice Timing - Reference Slice" be *180*? (i.e. either one of the two middle numbers in Slice order) (3) Can "fMRI model specification - Microtime resolution" be *500*? (as if there were 500 1-ms time-bins) (4) Can "fMRI model specification - Microtime onset" be *180*? (reference slice was acquired at the 180th 1-ms time-bin) The second study had TR = 2000 ms, and acquisition times below: >> hdr2 = spm_dicom_headers(xDicomFile{2}); >> hdr2{1}.Private_0019_1029' ans = 1.0e+03 * 0 0.9075 1.8150 0.7425 1.6500 0.5775 1.4850 0.4125 1.3200 0.2475 1.1550 0.0825 0.9900 1.8975 0.8250 1.7325 0.6600 1.5675 0.4950 1.4025 0.3300 1.2375 0.1650 1.0725 0 0.9075 1.8150 0.7425 1.6500 0.5775 1.4850 0.4125 1.3200 0.2475 1.1550 0.0825 0.9900 1.8975 0.8250 1.7325 0.6600 1.5675 0.4950 1.4025 0.3300 1.2375 0.1650 1.0725 0 0.9075 1.8150 0.7425 1.6500 0.5775 1.4850 0.4125 1.3200 0.2475 1.1550 0.0825 0.9900 1.8975 0.8250 1.7325 0.6600 1.5675 0.4950 1.4025 0.3300 1.2375 0.1650 1.0725 So again these numbers go to "Slice Timine - Slice order", and TR set to 2. Then: (1) Should "Slice Timing - TA" be *1.8975*? (i.e. max of Slice order) (2) Can "Slice Timing - Reference Slice" be *907.5*? (i.e. either one of the two middle numbers in Slice order) (3) Can "fMRI model specification - Microtime resolution" be *2000*? (as if there were 2000 1-ms time-bins) (4) Can "fMRI model specification - Microtime onset" be* 907*? (reference slice was acquired "approximately" at the 907th 1-ms time-bin) Any advice is greatly appreciated!!! Best Regards, Zhenhao -- Zhenhao Shi, Ph.D. Postdoctoral Fellow Center for Studies of Addiction, Department of Psychiatry University of Pennsylvania Perelman School of Medicine