Print

---

I was going to suggest the same but since it has already been said, here’s a cheeky suggestion: you could try determining the crystal structure of the complex and get a direct sequence readout for the nuclei acid.

Best,

Debanu 

Hi Jobi,

Phenol/CHCl3 extraction, iPrOH precipitation and then nucleic acid sequencing.

Best regards,

Philippe

 

---

Philippe BENAS, Ph.D.

Laboratoire de Cristallographie et RMN Biologiques, UMR 8015 CNRS
Faculté de Pharmacie, Université Paris Descartes
Case 48
Av, de l'Observatoire
F-75270 PARIS cedex 06
+33.1.5373.1599

E-mails: [log in to unmask], [log in to unmask]
URLs: http://lcrbw.pharmacie.univ-paris5.fr/ , http://lcrbw.pharmacie.univ-paris5.fr/spip.php?article18

---

---

De : Jobichen Chacko <[log in to unmask]>
À : [log in to unmask]
Envoyé le : Mardi 6 mars 2018 5h11
Objet : [ccp4bb] Off-topic question

Dear All,

We are trying to identify/sequence a DNA/RNA fragment (around 100bp) which was co-purified along with our protein.

The expression was done in E.coli.

Any suggestions on how to do this.

Thank you.

Jobi