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Hi, I was going to suggest the same but since it has already been said, here’s a cheeky suggestion: you could try determining the crystal structure of the complex and get a direct sequence readout for the nuclei acid. Best, Debanu > On Mar 5, 2018, at 9:34 PM, Philippe BENAS <[log in to unmask]> wrote: > > Hi Jobi, > > Phenol/CHCl3 extraction, iPrOH precipitation and then nucleic acid sequencing. > > Best regards, > Philippe > > Philippe BENAS, Ph.D. > > Laboratoire de Cristallographie et RMN Biologiques, UMR 8015 CNRS > Faculté de Pharmacie, Université Paris Descartes > Case 48 > Av, de l'Observatoire > F-75270 PARIS cedex 06 > +33.1.5373.1599 > E-mails: [log in to unmask], [log in to unmask] > URLs: http://lcrbw.pharmacie.univ-paris5.fr/ , http://lcrbw.pharmacie.univ-paris5.fr/spip.php?article18 > > > > De : Jobichen Chacko <[log in to unmask]> > À : [log in to unmask] > Envoyé le : Mardi 6 mars 2018 5h11 > Objet : [ccp4bb] Off-topic question > > Dear All, > We are trying to identify/sequence a DNA/RNA fragment (around 100bp) which was co-purified along with our protein. > The expression was done in E.coli. > Any suggestions on how to do this. > Thank you. > Jobi > >