You could try nautilus in CCP4. Steve Dr Stephen Carr Research Complex at Harwell (RCaH) Rutherford Appleton Laboratory Harwell Oxford Didcot Oxon OX11 0FA United Kingdom Email [log in to unmask] tel 01235 567717 ________________________________ From: CCP4 bulletin board <[log in to unmask]> on behalf of Madhu Sudhan <[log in to unmask]> Sent: 20 March 2018 18:28:36 To: ccp4bb Subject: [ccp4bb] How to fit DNA in density map of protein-DNA complex Dear all, I have an X-ray diffraction data of a protein-DNA complex at 2.3A resolution. I refined the structure, modeled the protein and the R-free reached to 0.35. I am observing extra density for the bound DNA molecule. As I am solving a protein-DNA complex structure for the first time, 1) Could anyone suggest me whether any automated program available to fit the DNA molecule into the density. 2) Also suggest me the other easiest ways, if any, to fit a DNA molecule into the electron density map. Thanks and Regards, Madhusudhan This email and any attachments may contain confidential, copyright and or privileged material, and are for the use of the intended addressee only. If you are not the intended addressee or an authorized recipient of the addressee, please notify us of receipt by returning the e-mail and do not use, copy, retain, distribute or disclose the information in or attached to this email. Any views or opinions presented are solely those of the author and do not necessarily represent those of the Research Complex at Harwell. There is no guarantee that this email or any attachments are free from viruses and we cannot accept liability for any damage which you may sustain as a result of software viruses which may be transmitted in or with the message. We use an electronic filing system. Please send electronic versions of documents, unless paper is specifically requested. This email may have a protective marking, for an explanation, please see: http://www.mrc.ac.uk/About/informationandstandards/documentmarking/index.htm.