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Dear Madhu, Just use Coot since it should works Best, -- Rafael Molina, Associate Professor University of Copenhagen The Novo Nordisk Foundation Center for Protein Research Faculty of Health and Medical Sciences Blegdamsvej 3B Denmark My Research Gate profile <https://www.researchgate.net/profile/Rafael_Molina4/?ev=prf_highl> — > El 20 mar 2018, a las 19:28, Madhu Sudhan <[log in to unmask]> escribió: > > Dear all, > > I have an X-ray diffraction data of a protein-DNA complex at 2.3A resolution. I refined the structure, modeled the protein and the R-free reached to 0.35. I am observing extra density for the bound DNA molecule. As I am solving a protein-DNA complex structure for the first time, > > 1) Could anyone suggest me whether any automated program available to fit the DNA molecule into the density. > > 2) Also suggest me the other easiest ways, if any, to fit a DNA molecule into the electron density map. > > > > Thanks and Regards, > > Madhusudhan >