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In addition to the other useful advice offered, I’d also suggest determining if the whole complex or just the DNA fits in the asymmetric unit. Particularly if the crystals are hexagonal rods or plates, you might have DNA-only crystals.
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Phoebe A. Rice
Dept. of Biochem & Mol. Biol. and
Committee on Microbiology
https://voices.uchicago.edu/phoebericelab/
From: CCP4 bulletin board <[log in to unmask]> on behalf of Natalia O <[log in to unmask]>
Reply-To: Natalia O <[log in to unmask]>
Date: Friday, March 2, 2018 at 7:35 PM
To: "[log in to unmask]" <[log in to unmask]>
Subject: [ccp4bb] does 12 A diffraction worth optimization
I got crystals of protein-nucleic acid complex, rod-shape, reproducible, don’t visibly get damaged upon freezing; however they gave
diffraction only to about 12 A. I tried several crystals. My question is whether such crystals worth optimization. Clearly a 4A diffracting crystal could potentially be optimized to 3 – 2.5A, but if the diffraction that I am getting now is 12A it could suggest
that the system is so flexible that getting to 3A with this crystal form is not possible at all. I just wonder if there is any statistics or a rule of thumb about what initial diffraction worth optimization?
Thank you!
-Natalia