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Absolutely you should optimise! It's impossible to predict crystal behaviour. I had a 20 A crystal, and I set a new plate in the same reservoir with an additive screen and got a 3A crystal. It was probably a completely different crystal to be honest, lattice, etc, but one never knows, you just have to try. You can also try using the low res crystal as a seed. Plus a million other things. Paul Steven Miller (PhD) Postdoctoral Researcher University of Oxford Wellcome Trust Centre for Human Genetics Division of Structural Biology Roosevelt Drive Oxford OX3 7BN ---- Original message ---- >Date: Fri, 2 Mar 2018 17:34:18 -0800 >From: CCP4 bulletin board <[log in to unmask]> (on behalf of Natalia O <[log in to unmask]>) >Subject: [ccp4bb] does 12 A diffraction worth optimization >To: [log in to unmask] > > Hello, > > > > I got crystals of protein-nucleic acid complex, > rod-shape, reproducible, don’t visibly get damaged > upon freezing; however they gave diffraction only to > about 12 A. I tried several crystals. My question is > whether such crystals worth optimization. Clearly a > 4A diffracting crystal could potentially be > optimized to 3 – 2.5A, but if the diffraction that > I am getting now is 12A it could suggest that the > system is so flexible that getting to 3A with this > crystal form is not possible at all. I just wonder > if there is any statistics or a rule of thumb about > what initial diffraction worth optimization? > > Thank you! > > -Natalia