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So if I calculate "structure factors" from a map do I just use the map as is with the negative values included ? Eleanor On 15 March 2018 at 14:37, Ian Tickle <[log in to unmask]> wrote: > > Hi Eleanor > > Electron scattering factors can be negative for negative ions, > particularly at low d*. For a low-resolution map it means that the > electric potential is the opposite sign to what you expected. It's why you > often don't see ASP & GLU side-chains in low resolution EM maps. > > Cheers > > -- Ian > > On 15 March 2018 at 14:17, Eleanor Dodson <0000176a9d5ebad7-dmarc- > [log in to unmask]> wrote: > >> I am pig-ignorant about these ,, but this example has negative values as >> well as positive.. >> >> What does this mean? I thought a well phased map would be pretty well all >> positive.. >> >> Eleanor >> > >