Hi Paul, You can try to detach the stylet from the muscle by scrubing gently the muscle and then make a transversal cut in the mantle near the tip of the sylet and try to pull them out from that insertion carefully avoiding to break them. I stored my collection of octopus vulgaris stylets in two different solutions Ethanol+glicerol and 4% formaline, and I found out that the center of the stylet became darkened more quickly with the ethanol solution, so I would recommend 4% formaline. But yes, I suggest to measure and weight them before stored. All the best, Sílvia On Mon, Feb 5, 2018 at 11:40 PM, Paul Bennetts <[log in to unmask]> wrote: > Hi Everyone, > > > I recently obtained some *E. dofleini * mantles and beaks. I am beginning > to dissect out the stylets and just want to make sure I am not going to > dammage the outer layers in the process. What is the standard way for > removing the stylets? Should I just leave part of the muscle attached? > > > I plan on preserving the stylets in 70% ethanol as well. Will this cause > the stylet to shrink from dehydration and should I be measuring stylet > length width and weight before preservation? > > > Any advice would help. > > > Thank you, > > Paul Bennetts > -- Sílvia Lourenço »«» «»« »«» «»« »«» «»« »«» «»« «»« »«» «»« »«» «»« »«» «»« »«» «»« »«» «»« »«» «»« »«» PhD Marine Ecology CMC - Centro de Maricultura da Calheta Direção Regional de Pescas da Região Autónoma da Madeira CIIMAR - Centro Interdisciplinar de Investigação Marinha e Ambiental OOM - Observatório Oceânico da Madeira e-mail: [log in to unmask] [log in to unmask] phone: 00351 291820420 skype: silvial30