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>>> 02/21/18 3:30 PM >>> HI, we are trying to install relion (ubuntu). it did well when we tried to start and tried the tutorial data, it gave us the following error. we could not figure out what it could be. it would be of great if the expertise could help us WARNING: there was an error executing: /home/rc/relion-demo/executable/MotionCor2_1.0.4 -InMrc /home/rc/relion-demo/Micrographs/Falcon_2012_06_12-17_26_54_0_movie.mrcs -OutMrc MotionCorr/job002//home/rc/relion-demo/Micrographs/Falcon_2012_06_12-17_26_54_0.mrc -LogFile MotionCorr/job002//home/rc/relion-demo/Micrographs/Falcon_2012_06_12-17_26_54_0 -Bft 150 -PixSize 3.54 -OutStack 1 -Patch 5 5 -Trunc 0 -Kv 300 -FmDose 1 -InitDose 0 -Gpu 0 >> MotionCorr/job002//home/rc/relion-demo/Micrographs/Falcon_2012_06_12-17_26_54_0.out 2>> MotionCorr/job002//home/rc/relion-demo/Micrographs/Falcon_2012_06_12-17_26_54_0.err GPL Ghostscript 9.18: Unrecoverable error, exit code 1 Thanks in advance Kind Regards Chitra >>> Sjors Scheres <[log in to unmask]> 01/11/18 10:35 AM >>> Hi Pu, Perhaps you mean FSCwork/FSCtest, like we used here: http://dx.doi.org/10.1126/science.1249410 One can calculate FSC curves in many programs. We used relion_image_handler. You need to refine the model in one of your half-maps and then test it, i.e. calculate FSC of the model converted to map against that same half-map (FSCwork) and against the other half-map, which the model never saw (FSCtest). HTH, Sjors On 01/10/2018 05:34 PM, Pu Qian wrote: > Hi > > I was told that there is a new validation program package for cryo-em map > and its atomic model called CTFwork/CTFtest. Does anyone know where I can > get it? Its authors, papers or webpages? Thanks in advance. > > Best regards > > Dr. Pu Qian > The University of Sheffield > Sheffield, S10 2TN > UK > -- Sjors Scheres MRC Laboratory of Molecular Biology Francis Crick Avenue, Cambridge Biomedical Campus Cambridge CB2 0QH, U.K. tel: +44 (0)1223 267061 http://www2.mrc-lmb.cam.ac.uk/groups/scheres