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Hi all, I found some strange errors while practicing ˇ¶Single-particle processing in RELION-2.1ˇ·with RELION-2.1 stable. If I use the GUI, I seted every parameter according section 3.6 "Making templates for auto-picking" of this tutorial. But when I click the button "Run now !", an error message was popup wroted "ERROR: unknown error in /home/cuikl/opt/relion-2.1/src/ml_optimiser_mpi.cpp at line 120 (error-code 30)", I clicked the "continue" button and a window was poput like this : Then I printed the command line corresponding the GUI job by Clicking the button "Print command", and I run the command job directly, but an error also occured : ˇ°ERROR: unknown error in /home/cuikl/opt/relion-2.1/src/ml_optimiser_mpi.cpp at line 120 (error-code 30)ˇ±. I opened this cpp file and checked line 120: "HANDLE_ERROR(cudaGetDeviceCount(&devCount));" . So I guess maybe something wrong about cuda, the I canceled the --GPU parameter ,run the command line again, this job runs regularly. What I confused were what's the meanning of this window message" ERROR: empty field for continuation STAR file... " and what's wrong about cuda? The command is like that: -------------------------------------------------------------------------------- $mpirun -np 3 relion_refine_mpi --o Class2D/job006/run --i Extract/job005/particles.star --dont_combine_weights_via_disc --preread_images --pool 3 --ctf --iter 25 --tau2_fudge 2 --particle_diameter 200 --K 10 --flatten_solvent --zero_mask --oversampling 1 --psi_step 10 --offset_range 5 --offset_step 2 --norm --scale --j 2 --gpu "0" ---------------------------------------------------------------------------------- Did I said clearly? Thanks in advance. Best regards. Kunlei.