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Hi Doryen, Below is the MTF of the F3 in EC mode at 300kV. I doubt you would notice any effects in doing the MTF-correction for the F2 and F3 images separately. HTH, Sjors data_mtf_falcon3EC_300kv loop_ _rlnResolutionInversePixel _rlnMtfValue 0 0.9946620523 0.0078125 0.9941721198 0.015625 0.9933405959 0.0234375 0.9920990051 0.03125 0.9904073587 0.0390625 0.9882715243 0.046875 0.9857194222 0.0546875 0.9830733385 0.0625 0.980149646 0.0703125 0.9767609578 0.078125 0.9729185764 0.0859375 0.9684782653 0.09375 0.9629033019 0.101562 0.9557795848 0.109375 0.9470537833 0.117188 0.9367337199 0.125 0.9247717762 0.132812 0.9112638747 0.140625 0.8964165693 0.148438 0.8804268374 0.15625 0.8635025257 0.164062 0.845907608 0.171875 0.8277927087 0.179688 0.8090417959 0.1875 0.7895827537 0.195312 0.7696551837 0.203125 0.7496061464 0.210938 0.7295747621 0.21875 0.7094132266 0.226562 0.6888578339 0.234375 0.6676661795 0.242188 0.6457769578 0.25 0.6234762562 0.257812 0.6012950732 0.265625 0.5795288992 0.273438 0.5581988846 0.28125 0.537286765 0.289062 0.5168282443 0.296875 0.4968426909 0.304688 0.4774179996 0.3125 0.4586162607 0.320312 0.440240978 0.328125 0.4218642279 0.335938 0.4033969873 0.34375 0.3852298464 0.351562 0.3676275219 0.359375 0.3504100662 0.367188 0.3334435228 0.375 0.3169838412 0.382812 0.3013100236 0.390625 0.2864074127 0.398438 0.2724968196 0.40625 0.2598722282 0.414062 0.2484933526 0.421875 0.2376316284 0.429688 0.226867896 0.4375 0.2164394897 0.445312 0.2066642742 0.453125 0.1970632583 0.460938 0.1885111132 0.46875 0.1808814517 0.476562 0.1741910049 0.484375 0.1707070749 0.492188 0.1679556258 0.50000 0.166 On 02/05/2018 01:21 PM, Bubeck, Doryen A wrote: > > I am integrating data collected on a Falcon II and Falcon III and was > wondering if there was a way to apply the appropriate MTF to the > corresponding images during the postprocessing step in Relion? Both > datasets were imaged at 300keV. > > > I also noticed the question in the mailinglist archive about trying to > track-down the MTF for the Falcon III, but couldn't see a response. > > > Any help greatly appreciated! > > > Doryen > -- Sjors Scheres MRC Laboratory of Molecular Biology Francis Crick Avenue, Cambridge Biomedical Campus Cambridge CB2 0QH, U.K. tel: +44 (0)1223 267061 http://www2.mrc-lmb.cam.ac.uk/groups/scheres