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Hi Tian, It's data related. Likely your images drifted translationally too far, since they were initially picked off-center. Try re-extracting with re-centering enabled with the data star-file of the last iteration in the 2D classification that crashed. In the next RELION version this issue will be handled internally and the hard crash replaced with a warning. Best, Dari On Feb 6, 2018 3:12 PM, "Tian. Li" <[log in to unmask]> wrote: > Hi, > > I'm working on 2D classification by Relion. But I constantly got the error > message below: Then the calculation stops. > > WARNING: Exception (filteredSize == 0) handled by switchign to fail-safe > mode. > WARNING: Exception (filteredSize == 0) handled by switchign to fail-safe > mode. > WARNING: Exception (filteredSize == 0) handled by switchign to fail-safe > mode. > WARNING: Exception (filteredSize == 0) handled by switchign to fail-safe > mode. > WARNING: Exception (filteredSize == 0) handled by switchign to fail-safe > mode. > Array_byarray:different shapes (*) > File: /programs/x86_64-linux/relion/2.0.3_cu8.0/src/multidim_array.h > line:2899 > > I never see this error before. I'm wondering if this is related to GPU or > something else, and how to fix it. > > Thanks, > > Tian >