Next MX-proposal application deadline: March 01, 2018 is approaching
http://www.helmholtz-berlin.de/user/beamtime/proposals/index_en.html
Hereby we would like to invite the submission of new proposals for
MX-beamtime at the HZB-MX beamlines for the next beam time period
(08/2018-01/2019).
In order to apply for beamtime, please register at the HZB on-line
access tool "GATE" (https://www.helmholtz-berlin.de/pubbin/hzbgate)
and submit a new beam time application proposal.
HZB provides MX-beamtime at the three MX-beamlines BL14.1, BL14.2
and BL14.3. The three beamlines are equipped with state-of-the-art
instrumentation and are currently the most productive MX-stations
in Germany with over 300 PDB depositions annually. Beamtime is
granted based on the reviewed proposals and on reports from
previous research activities. Please make sure to include them
if available.
What's new: Due to a planned upgrade of the experimental endstation
BL14.3 will not be fully available. Details can be provided
upon request.
Experimental setup:
BL14.1:
- Photon flux: 1.4x10¹¹ Phot/sx100mAx0.05%BW at sample position
(0.1-1 sec exposure time per frame)
- User defined beam shaping from 50µm-100µm diameter possible
- Pilatus 6M detector, 141mm-680mm max. distance from the sample
- Microdiffractometer (MD2) with Mini-kappa goniometer MK3
- Automatic sample changer (CATS), 90 sample storage capacity
(SPINE-Pin & EMBL sample magazine compatibility)
- 32-core XEON-CPU server, with 10Gb uplink to Pilatus 6M
- Data collection control via MXCuBE
- EDNA
- Common MX-software installed including XDS, iMOSFLM, CCP4,
Phenix, SHELXC-D-E, etc.
- Automated data processing with XDSAPP
- Remotely controlled cryo-shutter for crystal annealing
- Pressure chamber for noble gas derivatization (Xe, Kr
available upon request)
- AMPTEK-XRF detector and XFEPLOT software available
We are also offering the hard- and software environment for
carrying out:
- UV-RIP experiments at BL14.1. For further information, please visit:
https://www.helmholtz-berlin.de/forschung/oe/np/gmx/ancillary-facilities/uvrip_en.html
BL14.2:
- Photon flux: 1.9x10¹¹ Phot/sx100mAx0.05%BW at sample position
(0.1-1 sec exposure time per frame)
- Pilatus 3S-2M detector with 1000 micron Si sensor thickness,
55mm-600mm distance from the sample
- Nanodiffractometer (DESY P11 design) and on-axis sample microscope
- User defined beam shaping from 30µm-150µm diameter possible
- GROB sample changer for SPINE and UNIPUCK support
- 60-core XEON-CPU server, with fibre channel SAN up-link data
processing environment
- Common MX-software installed including XDS, iMOSFLM, CCP4, Phenix,
SHELXC-D-E, etc.
- Automated data processing with XDSAPP
- Amptek XRF detector
- Pressure chamber for noble gas derivatization (Xe, Kr available
upon request)
- Ultra high performance stereo microscope Leica M205A, 20-255x zoom,
8 Mpixel CCD-camera
- UV-Microsprectrophotometer offline setup available
- AMPTEK-XRF detector and XFEPLOT software available
BL14.3:
- Photon flux: 4x10exp10 Phot/sx100mAx0.05%BW at sample position
(3-20 sec exposure time per frame)
- Rayonix MX-225 X-ray detector, 45mm-380mm distance from the
sample, 30 deg 2-Theta possible
- MARdtb goniometer
- 60-core XEON-CPU server, with fibre channel SAN up-link data
processing environment
- EDNA installed and available
- Common MX software installed including XDS, iMOSFLM, CCP4, Phenix,
SHELXC-D-E, etc.
- Automated data processing using XDSAPP
- Remotely controlled cryo-shutter for crystal annealing
- HC1c dehydration device installed (please specify HC1-beamtime
in your proposal if needed)
- Pressure chamber for noble gas derivatization (Xe, Kr available
upon request)
- Ultra high performance stereo microscope Leica M205A, 20-255x zoom,
8 Mpixel CCD-camera
S1-biolab facilities (separate registration required):
- Protein production and purification
- Nanoliter 96 well crystallization plate formulation and storage at
5 °C and 20 °C
- Biophysical characterization with real time PCR (thermofluor assay)
The HZB-MX group is also providing expert assistance as well as
access to a library of fragments for carrying out crystallographic
fragment-screening experiments. For more information please contact
[log in to unmask].
Please visit our web page
www.helmholtz-berlin.de/bessy-mx to obtain
updated information about our experimental setup and other
requirements.
Manfred Weiss and the HZB-MX team
--
Dr. Manfred S. Weiss
Macromolecular Crystallography
Helmholtz-Zentrum Berlin
Albert-Einstein-Str. 15
D-12489 Berlin
Germany
Helmholtz-Zentrum Berlin für Materialien und Energie GmbH
Mitglied der Hermann von Helmholtz-Gemeinschaft Deutscher Forschungszentren e.V.
Aufsichtsrat: Vorsitzender Dr. Karl Eugen Huthmacher, stv. Vorsitzende Dr. Jutta Koch-Unterseher
Geschäftsführung: Prof. Dr. Bernd Rech (kommissarisch), Thomas Frederking
Sitz Berlin, AG Charlottenburg, 89 HRB 5583
Postadresse:
Hahn-Meitner-Platz 1
D-14109 Berlin
http://www.helmholtz-berlin.de
