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Dear Experts, Sorry for repeating this question again. I was doing fslvbm_3_proc, but by mistake I kept my design files in different folder. Since files were not available in the working directory it has thrown some error message. Please have a look and give your suggestions how to proceed further? Final steps from terminal; Doing registrations: ID=8848 /bin/cp: cannot stat '../design.*': No such file or directory ERROR: Program failed An exception has been thrown Unable to open design.mat Exiting ERROR: Program failed An exception has been thrown Unable to open design.mat Exiting ERROR: Program failed An exception has been thrown Unable to open design.mat Exiting Doing subject concatenation and initial randomise: ID= Once this has finished, run randomise with 5000 permutations on the 'best' smoothed 4D GM_mod_merg. We recommend using the -T (TFCE) option. For example: randomise -i GM_mod_merg_s3 -o GM_mod_merg_s3 -m GM_mask -d design.mat -t design.con -n 5000 -T -V Q2) When should I use the option -D in randomisation? after demean the covariate or with out demean the covariate? Thank you RB