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Hi Joshua, You may also want to consider splitting the data set in batches of say 100k particles for junk removal by class2D. There are some memory problems with large STAR files on some systems. The next release will stop these from happening. HTH, Sjors On 01/09/2018 10:16 PM, Joshua Lobo wrote: > Hi CCPEM > > I had a question regarding the --maxsig value for 2D Classification with the overall goal of weeding out junk particles and keeping iteration time down. Is it advisable to use the maxsig value when for the initial classification steps? I have a large dataset that I would like to classify ( greater than 1Million Particles) . I have binned the data to increase speed already. and am using the new subsets during first iterations mechanism in relion2.1 > > > Any suggestions are welcome > > Sincerely > Joshua Lobo -- Sjors Scheres MRC Laboratory of Molecular Biology Francis Crick Avenue, Cambridge Biomedical Campus Cambridge CB2 0QH, U.K. tel: +44 (0)1223 267061 http://www2.mrc-lmb.cam.ac.uk/groups/scheres