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Hi Simona, I have a problem that the particle will be aligned by wedge if apply no CTF file. As a result I just generated a binary wedge as a CTF file. It works perfect for me. The wedge was generated using TOM tool box. Best Qiang 2018-01-12 15:00 GMT+01:00 Simona Sorrentino <[log in to unmask]>: > Dear all, > > I acquired a dataset with the Volta Phase Plate, and I would like to do > subtomogram averaging from this data, without CTF correction, in RELION. > Are there any suggestions on how to prepare the 3D CTF models, for Phase > Plate data? > > Thanks in advance > > Simona > -- Qiang (John) Guo Department of Molecular Structural Biology Max Planck Institute of Biochemistry Am Klopferspitz 18 82152 Martinsried Germany Email: [log in to unmask]