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Hi Joshua, I typically find that 3D classification is reasonably smooth without maxsig (presumably the presence of a reference gives the particles something to latch onto a bit easier than in 2D). However when I feel its too slow, we use something between 500~2000. Within our group we typically do not recommend going below 500, but anything above 1000 is "very safe" as far as we are concerned. Best wishes, Shintaro <http://www.avg.com/email-signature?utm_medium=email&utm_source=link&utm_campaign=sig-email&utm_content=webmail> Virus-free. www.avg.com <http://www.avg.com/email-signature?utm_medium=email&utm_source=link&utm_campaign=sig-email&utm_content=webmail> <#DAB4FAD8-2DD7-40BB-A1B8-4E2AA1F9FDF2> On Tue, Jan 9, 2018 at 11:16 PM, Joshua Lobo <[log in to unmask]> wrote: > Hi CCPEM > > I had a question regarding the --maxsig value for 2D Classification with > the overall goal of weeding out junk particles and keeping iteration time > down. Is it advisable to use the maxsig value when for the initial > classification steps? I have a large dataset that I would like to classify > ( greater than 1Million Particles) . I have binned the data to increase > speed already. and am using the new subsets during first iterations > mechanism in relion2.1 > > > Any suggestions are welcome > > Sincerely > Joshua Lobo > -- Yours Sincerely, Shintaro Aibara