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Well - this is a pipeline where cycles of rebuilding are followed by cycles of refinement. The Freer is used during refinement - this is always a good idea - but you need to name the FreeRflag column. I think there is a default The selection "use map coefficient" and "use PHI/FOM instead of HL coefficient" are relevent for the Buccaneer cycles. First "use PHI/FOM instead of HL coefficient".. If you are starting from experimental phases (SAD or MAD) or parrot modification of these it is best to use HL coefficvients , but if you are rebuilding from a MR solution you may want to start from the 2mFOdFC and PHWT coefficents.. However if you provide the model the pipeline will begin with cycles of refinement and generate suitable map coeffoicients itself Eleanor On 13 January 2018 at 03:28, 张士军 <[log in to unmask]> wrote: > Dear all > > I am confusing how to choose or enter the labels when I do density > modification and autobuilding with ccp4 parrot and buccaneer or ARP/wARP, > there are three different options like "use Free R-flag ","use map > coefficient" and "use PHI/FOM instead of HL coefficient" in the figure I > attached. What they are mean, which one I should choose when I just want to > do density modification after SAD or MR,and how to do when autobuilding? > Even though when I choose"use map coefficient", it appeared > HLA,HLB,HLC,HLD,and when I choose "use PHI/FOM instead of HL coefficient", > it appeared PHI,FOM, how to enter these labels? Thanks a lot !!! > > Best Regards > > Shijun >