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Dear All, Wishing you all a very happy and prosperous new year 2018.... We have recently solved a structure which contains a huge density in the active site. We modelled a molecule fitting the electron density (please see the attached figures). However, we could not find any known molecule similar to the fitted molecule either in PDB or PubChem databases. Thus, we are not able to correlate its existence in cell. The protein was expressed in E. coli cells. The structure is solved at 1.85 Angst. We have not added any molecule like this in any step of the experiments. Looking forward to having some ways to figure out this problem. With best regards, Shankar [image: Inline image 1] -- Shankar Prasad Kanaujia, Ph.D. Associate Professor Department of Biosciences and Bioengineering Indian Institute of Technology Guwahati Guwahati - 781039 Assam, India Tele: 0361 258 2228 Fax: 0361 258 2249 Email: [log in to unmask] Homepage: http://www.iitg.ernet.in/spkanaujia/ Google Scholar: https://scholar.google.com/citations?user=Zt4JSNYAAAAJ&hl=en [image: Inline image 2]