To anyone analysing serum hCG on the Roche e411, We have a persistent positive bias on the hCG assay on the e411 using HCG+B lot 259199 and HCG+B CalSet lot 221092. We use third party controls and the low level IQC (target 10IU/L) runs fine, but our high level IQC (target 500IU/L) shows a persistent positive bias of ~10%. The 10% positive bias at concentrations >100 IU/L is also demonstrated in patient samples. We have seen the same issue on 3 different e411's. The IQC/EQA results are fine on the Cobas 6000 line using the same lot of reagent and calibrator. Would be interested to hear if anyone has observed the same issue with the e411. Many thanks, Sally Sally Willett Consultant Biochemist Blood Sciences North Cumbria University Hospitals NHS Trust, Cumberland Infirmary, CARLISLE, CA2 7HY. Tel: 01228 814521 Fax: 01228 814831 The NCUHT Pathology Handbook can be found here<http://www.ncuh.nhs.uk/for-gps/pathology/index.aspx> For general information on tests see Lab Tests Online<http://www.labtestsonline.org.uk/ > ******************************************************************************************************************** This message may contain confidential information. If you are not the intended recipient please inform the sender that you have received the message in error before deleting it. Please do not disclose, copy or distribute information in this e-mail or take any action in relation to its contents. To do so is strictly prohibited and may be unlawful. Thank you for your co-operation. NHSmail is the secure email and directory service available for all NHS staff in England and Scotland. NHSmail is approved for exchanging patient data and other sensitive information with NHSmail and other accredited email services. For more information and to find out how you can switch, https://portal.nhs.net/help/joiningnhsmail ------ACB discussion List Information-------- This is an open discussion list for the academic and clinical community working in clinical biochemistry. Please note, archived messages are public and can be viewed via the internet. Views expressed are those of the individual and they are responsible for all message content. ACB Web Site http://www.acb.org.uk Green Laboratories Work http://www.laboratorymedicine.nhs.uk List Archives http://www.jiscmail.ac.uk/lists/ACB-CLIN-CHEM-GEN.html List Instructions (How to leave etc.) http://www.jiscmail.ac.uk/