Hi FSL experts, I am getting different M0 value from asl_calib i.e.1.243, whereas oxford_asl gives M0 value=1.024. Below are command used for asl_calib: asl_calib -i perfusion -o perf_cal -s structural_brain --mode longtr --tissref csf --te 0.0159 --tr 4.55 --Mo Mo.txt -c calib -t asl2struct.mat Oxford_asl: oxford_asl -i diffdata --tis 3.2 -o oxford_wp -s structural_brain -t asl2struct.mat --casl --bolus 1.4 --fixbolus --report --te 0.0159 --tr 4.55 --cmethod voxel -c calib --alpha 0.85 -m mask --wp I want to know why there is differences in M0 values, calculated from asl_calib and oxford_asl and how is csf (reference tissue) used in calibration of perfusion map? Kindly help. Thank you, -- Archana V. Malagi, PhD Scholar, Centre for Biomedical Engineering, IIT Delhi-110 016 [log in to unmask]