Dear all,

While collecting FalconIII data in counting mode accidentally we got some images written out by FEI EPU as raw un-summed frames. I.e. instead of 40 fractions in file we got ~ 2000 frames. We would like to recover those files for further processing but the problem is that these huge files are written in 8 byte signed format and most programs we tried seem to treat it as unsigned (including Relion and Imod). In principle command "relion_image_handler --avg_bin 50 --i file --o file_av " should do what we like but it produces all 40 frames with minimal density = 0 and maximal ~3000, while in properly written by EPU files it ranges from -20 to ~ 600. So it looks like signed numbers are treated as unsigned.
Does anybody knows a way around this?
Many thanks!