Hello everyone, I have a very basic question regarding running ICA in FSL MELODIC. On the wiki page (https://fsl.fmrib.ox.ac.uk/fsl/fslwiki/MELODIC), it says: "Before calling the GUI, you need to prepare each session's data as a 4D NIFTI or Analyze format image; there are utilities in fsl/bin called fslmerge and fslsplit to convert between multiple 3D images and a single 4D (3D+time) image." I have DICOM files from my participants (260 for functional BOLD, per participant, per session). When I convert them to NIFTI format using dcm2nii software, I get a single NIFTI file. I would then assume that this is already 4D and I don't need to use fslmerge? Or should I merge these NIFTI files for every participant in my sample into one big NIFTI file? (But then this would not be 3d + time, but 3D+time+participants). (If it's relevant, I am trying to compare resting state functional connectivity features between a patient and control group, each participant has one session.) Thanks in advance for any help! Kind regards, Darko