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Hi, I've been searching but can't find what I am looking for so I thought I ask specialists. I am curious about the link between resolution limits of reflections on the detector, and what features are ordered in real space. I saw the great movie by James Holton on resolution and features in the electron density map, but I am looking for something more general. I am thinking that a reflection on the detector originates from something ordered within the crystal. The level of order would be different at different resolution. If you can help me fill the void in this phrase: I see spots at __A resolution, therefore I know that _____ features are ordered in my crystal. intuitively, I would build the following scale: 20A : the envelope is ordered 10A: a finer envelope is ordered 6A: alpha helices are ordered 4-5A: beta sheets are ordered and some residues 3-4A: residues start to be ordered >3A: more and more order. Has this been described somewhere? I would appreciate any comments and reevaluation of this scale. Thank you in advance. All the best Vincent -- Vincent Chaptal, PhD Institut de Biologie et Chimie des Protéines Drug Resistance and Membrane Proteins Laboratory 7 passage du Vercors 69007 LYON FRANCE +33 4 37 65 29 01 http://www.ibcp.fr