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Hi Herman,

I worked on a kinase, where moved from 6-His in the literature to 8-His, and it didn't impact crystallization or diffraction (which around 3Angs).

Good Luck
Partha
On Tue, Sep 19, 2017 at 9:43 AM Oganesyan, Vaheh <[log in to unmask]> wrote:

Hi Herman,

 

I haven’t done His-6 versus His-10 for the same protein, but have done that for different ones with success. However, if in His-6 containing protein structure the packing or folding is such that you don’t see His-6 then it shouldn’t matter it is 6 or 10. Just an opinion.

 

Regards,

 

Vaheh Oganesyan

www.medimmune.com

 

From: CCP4 bulletin board [mailto:[log in to unmask]] On Behalf Of [log in to unmask]
Sent: Tuesday, September 19, 2017 6:11 AM
To: [log in to unmask]
Subject: [ccp4bb] His-6 versus His-10 tag

 

Dear BB,

 

We are planning the production of a protein for crystallization. From literature, we know that the construct with a 6-histidine tag crystallizes. However, for other biophysical measurements, we would prefer to have a 10-histidine tag.

 

Does anyone has experience with His-6 versus His-10 tags in terms of crystallization success?

 

Thanks for your help!

Herman

 

 

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