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Thanks Anderson, No, age doesn't change between two time points but the two studied groups are significantly different in age and I want to control for that. Does the method that you mentioned earlier work for my actual problem? Thanks, Sara ________________________________ From: FSL - FMRIB's Software Library [[log in to unmask]] on behalf of Anderson M. Winkler [[log in to unmask]] Sent: Wednesday, August 09, 2017 6:22 AM To: [log in to unmask] Subject: Re: [FSL] two-way Mixed effect ANOVA Hi Sara, Is there a time lag > 1 year between pre and post intervention? That is, does the subjects' ages change substantially between the two timepoints? If not, then there is no need to include age (or any other factor that doesn't change, such as sex) in this design, as this is all accommodated by the subject-specific EVs. If however, age does change substantially between the two timepoints, then add an extra EV with the ages, but multiply the age at first timepoint of all subjects by -1 before putting in the design. All the best, Anderson On 9 August 2017 at 00:51, [log in to unmask]<mailto:[log in to unmask]> <[log in to unmask]<mailto:[log in to unmask]>> wrote: FSL Team, I want to compare pre and post intervention in two groups in TBSS. I am running two-way Mixed effect ANOVA as in https://fsl.fmrib.ox.ac.uk/fsl/fslwiki/GLM#Two_Groups_with_continuous_covariate_interaction and I want to have age as covariate. How should I design the gui to have the covariate. Thanks, Sara