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Hi This is a difficult tract to reconstruct and requires very good quality data and plenty of care in designing the a priori masks. Have a look at this paper where we were able to reconstruct the superior peduncle using HCP data: http://www.sciencedirect.com/science/article/pii/S1053811915008113 Perhaps you could try a similar strategy, and also reconstruct Cb-Thalamus and Thalamus-Cortex separately? Cheers, Saad On 8 Aug 2017, at 02:37, [log in to unmask]<mailto:[log in to unmask]> <[log in to unmask]<mailto:[log in to unmask]>> wrote: Hi FSL team, I am trying to trace the Cerebella-Thalamic-Cerebral tract in a group of children with a neurodevelopment disorder called Developmental Coordination Disorder (DCD). I use the following ROIs extracted from freesurfer for each participant: Seed point: left cerebellum WM Waypoints: left Superior Cerebellar Peduncle, Right Thalamus, and Right BA6+BA4 Exclusion Masks: CC+right cerebellum+right posterior limb of internal capsule I also force waypoints crossing in the listed order. For most of my participants, I get 0 or very few waytotals. Is it anything, I am doing wrong or it is normal. Thanks, Sara