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Dear Rohit, in your refinement table I find that the R-factors are elevated in the ice ring ranges (around 2.22 and 1.93 A resolution). And your data collections statistics seem to confirm this - CC1/2 is very high at high resolution which is often due to ice rings. Could be a classical case of "data collection problem becomes visible at refinement stage". best, Kay On Tue, 15 Aug 2017 22:09:44 +0530, rohit kumar <[log in to unmask]> wrote: >Dear All, > > > >I am refining a data in refmac5 and the data resolution is 1.8 A. Data is >looking fine with the data statics see below for data statics > > > >[image: Inline image 1] > > > >Right now the R/Rfree is 22/26 not good for such resolution. I have tried >most of the options in refmec5 but still I am not able to lower these >R/Rfree values. > >During refinement with phenix what I found that at resolution 1.93 to 1.86 >R/Rfree is quite high see below > > >[image: Inline image 2] > > > > >Is this the possible reason for high R/Rfree value?. If it is please let >me know how some can remove these frames during refinement. or > > >Please tell me other strategies to lower down the R/Rfree values. > > >Thank you in advance > > >With Regards > >Dr. Rohit Kumar Singh >