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Hi Nora, Are you centrifuging as per Sarstedt recommendations for the longer time or higher spin speed for the plasma gel tube? Extract from Sarstedt protocols "In order to achieve an evenly formed separating layer, centrifugation must be carried out in a centrifuge with a swing-out rotor. The S-Monovette(r) Serum-Gel is to be centrifuged at 2500 xg for 10 minutes and the S-Monovette(r) Plasma-Gel at 2500 xg for 15 minutes or 3000 xg for 10 minutes, both at room temperature. We have had fliers in plasma gel on Abbott Architect for Na, Alb, Alk and GGT as well as fT4 and we haven't been able to get to the true cause as yet. Our issue is not quite the same as you describe in that the repeat in the same plasma sample is different, but the coincidence is that it is broadly the same tests. Best, Maria Prof Maria Fitzgibbon, Consultant Clinical Biochemist/UCD Clinical Professor, Department of Clinical Biochemistry/Diagnostic Endocrinology, Mater University and Mater Private Hospitals/School of Medicine, University College Dublin, Eccles St, Dublin 7. Tel: 0035318032423 email: [log in to unmask]<mailto:[log in to unmask]> From: Clinical biochemistry discussion list [mailto:[log in to unmask]] On Behalf Of nora keogh Sent: 02 August 2017 13:02 To: [log in to unmask] Subject: Suspect pattern of results Hi guys, Just querying has anybody come across this unusual pattern of blood results, see below. I suspect its preanalytical - Albumin, Sodium, Potassium, and ALK are affected. We received a Lithium Heparin and Serum sample on the same patient in the same delivery. There is some interference with the Lithium Heparin sample that is not affecting the serum sample. Samples were assayed x2 on two different days and we retrieved the same result!! Any ideas!! Analyte Lithium Heparin Tube (Sarstedt) ( B,101953) Sample Date and Processed 31/05/17 Serum Tube (Sarstedt) (B,101952) Sample Date and Processed 31/05/17 *Serum Tube (B,101952) Sample Date: 31/05/17 Processed 01/06/17 (Day Old) Sodium 132 mmol/L 132mmol/L NA 143 mmol/L 144 mmol/L Potassium 6.7 mmol/L NA 4.7 mmol/L 4.71 mmol/L Chloride 102 mmol/L 102 mmol/L NA 104 mmol/L Urea 3.8 mmol/L NA 4.0 mmol/L Creatinine 83 umol/L NA 86 mmol/L Calcium 2.274 mmol/L (R101953) NA 2.353 mmol/L Total Protein 74 g/L NA 73 g/L Albumin 6 g/L 01/06/17 8.8g/L (Cup) NA 46 g/L 45 g/L Bilirubin 10 umol/L NA 5.1 umol/L GGT 64 U/L NA 67 U/L ALK 75 U/L NA 84 U/L ALT 52 U/L NA 52 U/L Cholesterol 5.3 mmol/L NA 5.4 mmol/L Triglyceride 1.3 mmol/L NA 1.4 mmol/L Cholesterol- HDL 1.1 mmol/L NA 1.1 mmol/L cLDL 3.6 mmol/L NA 3.8 mmol/L FT4 13.6 pmol/L NA 13.2 pmol/L TSH 1.13 mU/L NA 1.2 mU/L Serum Index H 57 NA 10 Serum Index I 1 NA 1 Serum Index L 13 NA 5 Lithium NA 0.68 mmol/L NA Kind Regards, Nora Keogh, Chief Medical Scientist, Clinical Chemistry Laboratory, Naas General Hospital, *045 843044 ------ACB discussion List Information-------- This is an open discussion list for the academic and clinical community working in clinical biochemistry. Please note, archived messages are public and can be viewed via the internet. Views expressed are those of the individual and they are responsible for all message content. ACB Web Site http://www.acb.org.uk Green Laboratories Work http://www.laboratorymedicine.nhs.uk List Archives http://www.jiscmail.ac.uk/lists/ACB-CLIN-CHEM-GEN.html List Instructions (How to leave etc.) http://www.jiscmail.ac.uk/ This e-mail and any files transmitted with it contain information which may be confidential and which may also be privileged and is intended solely for the use of the individual or entity to which it is addressed. Unless you are the intended recipient you may not copy or use it, or disclose it to anyone else. Any opinions expressed are that of the individual and not necessarily that of Mater Misericordiae University Hospital. ------ACB discussion List Information-------- This is an open discussion list for the academic and clinical community working in clinical biochemistry. Please note, archived messages are public and can be viewed via the internet. Views expressed are those of the individual and they are responsible for all message content. ACB Web Site http://www.acb.org.uk Green Laboratories Work http://www.laboratorymedicine.nhs.uk List Archives http://www.jiscmail.ac.uk/lists/ACB-CLIN-CHEM-GEN.html List Instructions (How to leave etc.) http://www.jiscmail.ac.uk/