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Hi Wayne, I did add the file in the directory as you mentioned and restarted the system too. Stil I am getting the same error. On Fri, Jul 21, 2017 at 9:24 PM, Wayne Boucher <[log in to unmask]> wrote: > Did you add the xml file to that directory and restart Analysis? > > Wayne > > On 21 Jul 2017, at 16:53, madhan kumar <[log in to unmask]> wrote: > > Dear Wayne, > I am getting the following error message after I added NH2 at the end of > the sequence. > > Residue code(s) NH2 cannot be found for molecule type protein. > > > On Fri, Jul 21, 2017 at 9:02 PM, madhan kumar <[log in to unmask]> wrote: > >> Hi Wayne, >> >> Thank so much. I will try as you mentioned and get back you. >> >> Thanks, >> Madhan Kumar >> >> On Fri, Jul 21, 2017 at 8:55 PM, Wayne Boucher <[log in to unmask]> wrote: >> >>> Hello, >>> >>> Fortunately Tim just passed through so he showed me how to create this >>> NH2 as if it were an amino acid, so with the correct link to the “previous” >>> residue. So I’ve managed to create an NH2 xml ChemComp file of type >>> “protein”, which I’ve attached. >>> >>> Copy that file into your release directory into the subdirectory >>> ccpnmr2.4/data/ccp/molecule/ChemComp/. Then in the Molecule —> >>> Molecules dialog go to the Add Sequence tab and in 3-letter/Ccp mode you >>> can add NH2 at the end of your sequence. >>> >>> Hopefully that will work. (It seems to work for me but I haven’t gone >>> much past the above.) >>> >>> Wayne >>> >>> >>> On 21 Jul 2017, at 16:06, madhan kumar <[log in to unmask]> wrote: >>> >>> Hi Brian, >>> >>> Yes. I did. My question is this: If my protein is having C terminal >>> amidation, how can I add NH2 at the C terminal when I am doing "Add >>> Sequence".? >>> >>> On Fri, Jul 21, 2017 at 4:56 PM, Brian Smith <[log in to unmask]> >>> wrote: >>> >>>> Hi Madhan, >>>> >>>> Have you tried copying your existing assignments to the new chain that >>>> you think you have created? If not give that a go first. >>>> >>>> 1. Archive your project! >>>> 2. follow: >>>> >>>> https://sites.google.com/site/ccpnwiki/Home/documentation/cc >>>> pnmr-analysis/howtos-1/copy-assignments-between-molecular-chains >>>> >>>> If this does not succeed, you haven't yet finished making your modified >>>> molecule - go back to >>>> >>>> https://sites.google.com/site/ccpnwiki/Home/documentation/cc >>>> pnmr-analysis/howtos-1/create-a-sequence-with-unusual-amino- >>>> acids-or-nucleotides >>>> >>>> >>>> Dr. Brian O. Smith --------------------------- Brian Smith at glasgow >>>> ac uk >>>> Institute of Molecular, Cell and Systems Biology & School of Life >>>> Sciences, >>>> College of Medical, Veterinary & Life Sciences, >>>> Joseph Black Building, University of Glasgow, Glasgow G12 8QQ, UK. >>>> Tel: 0141 330 5167/6459/3089 >>>> ---------------------------------------------------------------------- >>>> The University of Glasgow, charity number SC004401 >>>> ------------------------------ >>>> *From:* CcpNmr software mailing list [[log in to unmask]] on behalf >>>> of madhan kumar [[log in to unmask]] >>>> *Sent:* 20 July 2017 14:21 >>>> *To:* [log in to unmask] >>>> *Subject:* Assignment of C terminal amidated protein >>>> >>>> Dear all, >>>> >>>> I am using Analysis 2.4.2. I have a protein which is C-terminal >>>> amidated. I did assignments for the protein without adding Nh2 at first in >>>> sequence. So I have created a new molsystem and new chain with the same >>>> sequence along with Nh2 at c terminal. I am having a problem to assign the >>>> Nh2 in 15N HSQC spectra. I picked the peak and gone for assignments. I did >>>> click assign but I couldn't see the newly added Molsystem at all. May I >>>> know how to do assignment for the same? >>>> >>>> Thank you in advance. >>>> >>>> -- >>>> Thanks & Regards, >>>> M.Madhan Kumar, >>>> Molecular Biophysics Unit, >>>> Indian Institute of Science, >>>> Bangalore. India. >>>> +91 8277200969 >>>> >>> >>> >>> >>> -- >>> Thanks & Regards, >>> M.Madhan Kumar, >>> Molecular Biophysics Unit, >>> Indian Institute of Science, >>> Bangalore. India. >>> +91 8277200969 >>> >>> >>> >>> >> >> >> -- >> Thanks & Regards, >> M.Madhan Kumar, >> Molecular Biophysics Unit, >> Indian Institute of Science, >> Bangalore. India. >> +91 8277200969 >> > > > > -- > Thanks & Regards, > M.Madhan Kumar, > Molecular Biophysics Unit, > Indian Institute of Science, > Bangalore. India. > +91 8277200969 > > > -- Thanks & Regards, M.Madhan Kumar, Molecular Biophysics Unit, Indian Institute of Science, Bangalore. India. +91 8277200969