Have to do primer walking in a cdna library first.

Artem

On Jul 24, 2017 7:26 AM, "syed ibrahim" <[log in to unmask]> wrote:
Hello All

I am interested in cloning a gene from a plant. I searched the database only partial sequence is available, ie: for 160 residues only. The full length of the protein is around 570 residues. I designed forward primer and I have no clue to design reverse primer.

Any help

Thank you

Syed