You've got multiple lattices--try seeding approaches mentioned in a recent/current thread.
JPK
-----Original Message-----
From: CCP4 bulletin board [
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Sent: Thursday, July 13, 2017 3:56 AM
To:
[log in to unmask]Subject: [ccp4bb] weird diffraction pattern
hello everyone,
I would like to seek your opinion on my crystal hits. I am working on a helicase
of which the native structure is solved and the all solution statistics are
fine. I am trying to crystallize and solve the structure of the protein/ssDNA
complex. I recently got some hits from commercial screens using sitting drop
vapor diffusion. After crystallization optimization, these crystals diffract
weakly but to 3.2 Angstroms for the longer exposure time. However, when the
crystals rotate between 120 degrees to 180 degrees, the spots become streaky
(attached), no matter the crystals are hexagonal or flaky. I have tried to
determine the structure by molecular replacement method, but the Rwork/Rfree
values are huge (above 0.5) and can’t be reduced further. I suspect the
obtained crystals quality and resulting processed statistics is the reason for
the observed high Rwork/Rfree values. Are there any suggestions?
All comments will be appreciated!
Best,
Chenjun Tang