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Have to do primer walking in a cdna library first. Artem On Jul 24, 2017 7:26 AM, "syed ibrahim" < [log in to unmask]> wrote: Hello All I am interested in cloning a gene from a plant. I searched the database only partial sequence is available, ie: for 160 residues only. The full length of the protein is around 570 residues. I designed forward primer and I have no clue to design reverse primer. Any help Thank you Syed