Hi Marco, but what's the best way if the first volume is severely distorted by motion during the scan? The first realignment step would use a bad volume with unpredictable results on the final mean reference. Doing the first step with a good reference could be a solution. But how can I do it in SPM? Cheers, Bertram On Tue, 27 Jun 2017 11:00:32 +0200, Marko Wilke <[log in to unmask]> wrote: > Hi Bertram, > > in the batch interface, there is actually a setting for this ("Register > to first" or "Register to mean"). In the latter case, there will > basically be a two step procedure: registering all images to the first, > create mean, then register all images to this one. You should also be > able to implement this on the command line, passing a 'rtm' flag to > spm_realign. In neither case should you have to manually edit the file > order to achieve this. > > Cheers > Marko > > Bertram Walter wrote: >> Dear all, >> >> I want to use the middle volume of my functional scan as reference scan >> for realignment in realign & unwarp. In the manual it is mentioned >> >> "The first image in the list specified by the user is used as a reference >> to which all subsequent scans are realigned. The reference scan does not >> have to the the first chronologically and it may be wise to chose a >> "representative scan" in this role." >> >> My question: How do I have to specify the scans in realign&unwarp? >> >> E.g. if I have 10 scans and want to use scan # 5 as reference >> >> Option A: When entering 10 scans in the sequence >> 5 1 2 3 4 6 7 8 9 10 >> I get realignment parameters for 10 volumes >> >> 0.0000000e+00 -1.4331259e-14 1.2492012e-14 9.6730144e-19 >> 1.1186837e-16 2.6148260e-17 >> 1.2853422e-01 8.9730393e-02 3.6775770e-02 -9.4771013e-06 >> -3.2223731e-04 1.4239692e-03 >> 4.6301388e-02 -5.0520113e-02 -6.9045253e-02 -6.6616240e-04 >> -2.1641583e-05 5.6118550e-04 >> 4.6174405e-02 7.0661590e-02 8.4805501e-02 3.6093835e-04 >> 3.1108790e-04 8.4482913e-05 >> 2.1246018e-02 1.2126505e-02 2.8272003e-02 -9.6548672e-04 >> -2.1787263e-04 2.5528354e-04 >> 4.2798897e-02 9.4051293e-02 1.7265070e-01 -1.0246176e-03 >> -1.2925461e-04 2.8213895e-04 >> 1.1362071e-02 6.6483921e-03 7.1757735e-02 -8.6840762e-04 >> -1.9301134e-04 -2.7897976e-04 >> 9.8239930e-03 8.1840421e-02 1.2797305e-01 5.9959604e-04 >> 2.9641170e-04 -5.0873017e-04 >> 1.4794704e-02 9.1027866e-02 9.7297841e-02 5.4889707e-04 >> -9.8659697e-05 -5.5278722e-04 >> 9.3970797e-03 1.1272641e-01 1.2209011e-01 3.9738355e-04 >> 5.6547393e-05 -8.0964723e-04 >> >> The first line refers to scan no 5, right? >> The resampled nifti contains 10 volumes >> >> Option B: When entering 11 scans in the sequence >> 5 1 2 3 4 5 6 7 8 9 10 >> I get realignment parameters for 11 volumes >> >> 0.0000000e+00 -1.4331259e-14 1.2492012e-14 9.6730144e-19 >> 1.1186837e-16 2.6148260e-17 >> 1.2853422e-01 8.9730393e-02 3.6775770e-02 -9.4771013e-06 >> -3.2223731e-04 1.4239692e-03 >> 4.6301388e-02 -5.0520113e-02 -6.9045253e-02 -6.6616240e-04 >> -2.1641583e-05 5.6118550e-04 >> 4.6174405e-02 7.0661590e-02 8.4805501e-02 3.6093835e-04 >> 3.1108790e-04 8.4482913e-05 >> 2.1246018e-02 1.2126505e-02 2.8272003e-02 -9.6548672e-04 >> -2.1787263e-04 2.5528354e-04 >> -1.1166691e-14 -5.5177401e-15 1.2492012e-14 -5.7451305e-17 >> 2.3912931e-16 2.4868550e-17 >> 4.2798897e-02 9.4051293e-02 1.7265070e-01 -1.0246176e-03 >> -1.2925461e-04 2.8213895e-04 >> 1.1362071e-02 6.6483921e-03 7.1757735e-02 -8.6840762e-04 >> -1.9301134e-04 -2.7897976e-04 >> 9.8239930e-03 8.1840421e-02 1.2797305e-01 5.9959604e-04 >> 2.9641170e-04 -5.0873017e-04 >> 1.4794704e-02 9.1027866e-02 9.7297841e-02 5.4889707e-04 >> -9.8659697e-05 -5.5278722e-04 >> 9.3970797e-03 1.1272641e-01 1.2209011e-01 3.9738355e-04 >> 5.6547393e-05 -8.0964723e-04 >> >> The first line and the sixth line refer to scan no 5, right? >> The resampled nifti contains again 10 volumes and differs slightly from >> the nifti resulting from option A. >> >> Which version should be used? >> >> Many thanx, >> >> Bertram >> > -- Dr. Bertram Walter Bender Institute of Neuroimaging University of Giessen Otto-Behaghel-Str. 10H 35394 Giessen Germany Phone +49 (641) 99-26307 or +49 (641) 99-26331 (Secretary) Fax +49 (641) 99-26309 www.bion.de