 |
 |
Dear Michele, The second 'solvent' mask should only be used for the inside of non-empty shells (like viruses). It should not overlap with the first one. Sjors > Dear all, > > I am wondering how the overlapping volume is treated when the solvent mask > (--solvent_mask) and the secondary mask (--solvent_mask2) partially > overlap: is it considered part of the first mask, or part of the second? I > am using Relion 1.4. > > Should the reference density include also to the volume covered only by > the > secondary mask? > > > Best regards, > Michele > -- Sjors Scheres MRC Laboratory of Molecular Biology Francis Crick Avenue, Cambridge Biomedical Campus Cambridge CB2 0QH, U.K. tel: +44 (0)1223 267061 http://www2.mrc-lmb.cam.ac.uk/groups/scheres