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We have sometimes used Michael Garavito's excellent suggestion of diffusing glutaraldehyde into the drops - mentioned on this board a few days ago. Our protein crystals either turned brown or turned into brown goo. Michael and others, what effect would you expect glutaraldehyde to have on DNA crystals? Patrick On 19 June 2017 at 15:20, Joseph Ho <[log in to unmask]> wrote: > Dear all: > > I would like to seek your opinion on our crystal hits. We are working > on protein/dsDNA complex. By changing different protein and DNA > (14-22bp) constructs, we recently got some hits from commercial > screens using sitting drop vapor diffusion (very small xtals). The > precipitant is PEG and the picture of crystals are attached. In this > particular condition, it is 30%PEG3350, sodium succinate pH5.5 and > 100mM NaCl. The crystal seems floating and sit in the bottom. We do > some test shot from other conditions and it is not salt crystals. The > crystals can suck in izit dye. I do some google and it seems izit dye > also turns dsDNA crystal into blue. We also do UV/Vis microscope but > no Trp fluorescence (6 Trp in 256 aa). It may due to low Trp. > > This is our first time to work on protein/DNA complex crystals and we > are not certain if this is just DNA or protein/DNA crystals. Can you > provide your comments on our hits? > > Thank you for your help > > Joseph > -- [log in to unmask] Douglas Instruments Ltd. Douglas House, East Garston, Hungerford, Berkshire, RG17 7HD, UK Directors: Peter Baldock, Patrick Shaw Stewart http://www.douglas.co.uk Tel: 44 (0) 148-864-9090 US toll-free 1-877-225-2034 Regd. England 2177994, VAT Reg. GB 480 7371 36