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Saad,

That seems to have fixed the error but the output folder only contains one output fdt_paths.nii.gz and the waytotal has only one number, both of which are similar to the output when running tractography without any condition. Am I missing something?

{FSLDIR}/bin/probtrackx2  -x $SEEDFILENAME --targetmasks=$TARGETSLIST -l --onewaycondition -c 0.2 -S 2000 --steplength=0.5 -P 5000 --fibthresh=0.01 --distthresh=0.0 --sampvox=0.0 --forcedir --opd -s {BEDPOSTX_FOLDER}/merged -m {BEDPOSTX_FOLDER}/nodif_brain_mask  --dir=${OUTPUT_FOLDER} --otargetpaths

Thanks,
/Suraj