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Hi Mani, Assuming you have images in MRC format, I think you may have a corrupt STAR file, where some image is being read in Spider format (i.e. with a non-mrc or .mrcs extension). HTH, Sjors > Hi All, > > I get the following error from a 3d focus classification run of signal > subtracted particles set without any alignment. > > ************** > Expectation iteration 1 of 25 > 49/ 49 sec > ............................................................~~(,_,"> > Maximization ... > 60/ 60 sec > ............................................................~~(,_,"> > ERROR: > rwSPIDER: cannot allocate memory for header > File: /programs/x86_64-linux/relion/2.0.4/src/rwSPIDER.h line: 134 > ************** > > The 2d classification of the same set of signal subtracted particles > works fine without any problem. > > Please help me to overcome this. > > with kind regards > > Mani. > -- Sjors Scheres MRC Laboratory of Molecular Biology Francis Crick Avenue, Cambridge Biomedical Campus Cambridge CB2 0QH, U.K. tel: +44 (0)1223 267061 http://www2.mrc-lmb.cam.ac.uk/groups/scheres