Print

Print
Hi Sebastian,

you're thinking about local sparse matrix screening.  I've done this at a 90:10 ratio.

Majeed, S., Ofek, G., Belachew, A., Huang, C.C., Zhou, T., and Kwong, P.D.
Enhancing protein crystallization through precipitant synergy.
Structure. 2003; 11: 1061–1070

All best.


Andreas



On Mon, May 8, 2017 at 3:18 PM, Sebastiano Pasqualato <[log in to unmask]> wrote:

Dear all,
I recall a paper (or was is a talk at a conference?) describing the optimisation of initial hits of crystallisation by using a standard screen kit as additive.
Something like setting the tray using the initial crystallisation hit condition in the reservoir and mixing 75% of the hit condition with 25% of a commercial sparse matrix screen kit with the protein in the drop.
I can’t find the reference, can anybody help me?
Thanks a lot,
ciao,
Sebastiano


-- 
Sebastiano Pasqualato, PhD
Crystallography Unit
Department of Experimental Oncology
European Institute of Oncology
IFOM-IEO Campus
via Adamello, 16
20139 - Milano
Italy

tel +39 02 9437 5167
fax +39 02 9437 5990